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“A key part of this study was to create a method to extract and analyze DNA from single hairs of prey species found in the teeth of historical museum specimens,” said lead study author Alida de Flamingh, postdoctoral research fellow at the University of Illinois Urbana-Champaign, in a statement. “Our analysis showed that the historic Tsavo lions preyed on giraffe, human, oryx, waterbuck, wildebeest, and zebra, and we also identified hairs that originated from lions. This method can be used in many ways, and we hope other researchers will apply it to study prey DNA from other animal skulls and teeth.”
Gnoske and his colleague and study coauthor Julian Kerbis Peterhans, an adjunct curator at the Field Museum and professor of natural science at Roosevelt University, have studied the lion skulls for decades.
Gnoske made the discovery that both lions were adult males, although both lacked the signature manes associated with fully grown males. A lack of mane in adult male lions is common and can occur based on the environment and climate where the animals live, injuries that occur when their manes are developing and other factors, he said.
Gnoske and Kerbis Peterhans also first reported on the damaged condition of the lions’ teeth in 2001, which might have played a partial role in why the animals shifted their focus to attack and eat humans. One of the lions may have sustained damage from a kick or a blow from a buffalo or zebra, which resulted in its inability to hunt normal prey efficiently, according to their research.
“We cannot claim that any single cause will guarantee that a lion will turn into a ‘maneater’, but it is clear that a variety of causes will increase the likelihood,” they wrote in theJanuary 2001 study, including a prey-depleted landscape or predators that already have a taste for humans after scavenging corpses.
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For the new study, Gnoske and Kerbis Peterhans carefully removed some of the hairs. The team focused on four tiny individual hairs and three hair clumps, all of which were well over 100 years old.
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“We were even able to get DNA from fragments that were shorter than the nail on your pinky finger,” de Flamingh said.
Hairs from the lions show that they shared the same maternally inherited mitochondrial genome, which supports earlier beliefs that the two males were siblings. And lion hair trapped in their teeth suggests that the brothers were closely bonded and groomed one another, Kerbis Peterhans said.
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“It suggests that the Tsavo lions may have either traveled farther than previously believed, or that wildebeest were present in the Tsavo region during that time,” de Flamingh said.
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“Patterson kept a handwritten field journal during his time at Tsavo,” Kerbis Peterhans said. “But he never recorded seeing buffalo or indigenous cattle in his journal.”
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“There may be descendants still in the region today and to practice responsible and ethical science, we are using community-based methods to extend the human aspects of the larger project,” the authors wrote in the study. “The anthropological methods require discussions with local institutions and groups about the project as well as reporting the detailed human colonial history of this geographic region, which is beyond the scope of this current study.”
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Love Dalén, a professor of evolutionary genomics at Stockholm University, considers the technique “quite ingenious.”
“We’ve known for some time that it’s possible to get dietary information by extracting DNA from dental plaque in ancient teeth,” said Dalén, who wasn’t involved in this study. “But no one has thought to instead use hairs in carnivore teeth as a source for DNA.”
“In the published literature at least, I don’t know that there are any individual lions in history to have had such a diverse and long list of prey species documented,” Gnoske said.
Future analysis of the layers of hairs will enable the team to reconstruct a partial timeline of the lions’ diet and determine when they began hunting humans.
“Layersin lower parts of the tooth cavity represent prey eaten earlier in life and layers at the top of the cavity are from recently eaten prey,” de Flamingh said. “This type of analysis can give insights into human-lion conflict,which is still impacting many communities in the region and broadly in Africa. For example, if lions begin by hunting wildlife but then start attacking domestic animals, and eventually turn to preying on humans, we can develop strategies and recommendations to reduce these risks.”
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